Publications

  1. Marshall R*, Maxwell CS*, Collins SP, Jacobsen T, Luo ML, Begemann MB, Gray BN, January E, Singer A, He Y, Beisel CL#, Noireaux V#. Rapid and scalable characterization of CRISPR technologies using an E. coli cell-free transcription-translation system. Mol Cell (accepted)
  2. Fagen JR, Collias D, Singh AK, Beisel CL. Advancing the design and delivery of CRISPR antimicrobials. Curr Opn Biomed Eng (2017) 4:57-64. article
  3. Waller MC, Bober JR, Nair NU, Beisel CL. Toward a genetic tool development pipeline for host-associated bacteria. Curr Opin Microbiol (2017) 38:156-164. pubmed article
  4. Marshall R, Maxwell CS, Collins SP, Beisel CL, Noireaux V. Short DNA containing Chi sites enhances DNA stability and gene expression in E. coli cell-free transcription-translation systems. Biotechnol Bioeng (2017) 114(9):2137-41. pubmed article
  5. Leenay RT & Beisel CL. Deciphering, communicating, and engineering the CRISPR PAM. J Mol Biol (2017) 429(2):177-91. pubmed article
  6. Luo ML, Beisel CL. SBE Supplement: Synthetic Biology – Engineering Genes with CRISPR-Cas9. Chemical Engineering Progress. September 2106 issue. article
  7. Luo ML, Jackson RN, Denny SR, Tokmina-Lukaszewska M, Maksimchuk KR, Lin W, Bothner B, Wiedenheft B, Beisel CL. The CRISPR RNA-guided surveillance complex in Escherichia coli accommodates extended RNA spacers. Nucleic Acids Res (2016) 44(15):7385-94 pubmed article
  8. Leenay RT, Maksimchuk KR, Slotkowski RA, Agrawal RN, Gomaa AA, Briner AE, Barrangou R#, Beisel CL#. Identifying and visualizing functional PAM diversity across CRISPR-Cas systems. Mol Cell (2016) 62(1):137-47 pubmed article
  9. Luo ML, Leenay RT, Beisel CL. Current and future prospects for CRISPR-based tools in bacteria. Biotechnol Bioeng (2016) 113(5):930-43. pubmed article
  10. Beisel CL & Afroz T. Rethinking the hierarchy of sugar utilization in bacteria. J Bacteriol (2015) 198(3):374-6 pubmed article
  11. Sun, W, Ji W, Hall JM, Hu Q, Wang C, Beisel CL#, Gu Z#. Efficient delivery of CRISPR-Cas9 for genome editing via self-assembled DNA nanoclews. Angew Chem Int Ed (2015) 54(41):12029-33 pubmed article See comment in Chemical & Engineering News, Genetic Engineering & Biotechnology News, WRAL news
  12. Afroz T, Luo ML, Beisel CL. Impact of residual inducer on titratable expression systems. PLoS One (2015) 10(9):e0137421. pubmed article
  13. Luo, ML, Mullis AS, Leenay RT, Beisel CL. Repurposing endogenous Type I CRISPR-Cas systems for programmable gene repression. Nucleic Acids Res (2015) 43(1):674-81 pubmed article See comment in BD Accuri News
  14. Afroz T, Biliouris K, Boykin KE, Kaznessis Y, Beisel CL. Trade-offs in engineering sugar utilization pathways for titratable control. ACS Synth Biol (2015) 4(2):141-9. pubmed article
  15. Briner AE*, Donohoue PD*, Gomaa AA*, Selle K, Slorach EM, Nye CH, Haurwitz RE, Beisel CL#, May AP#, Barrangou R#. Guide RNA functional modules direct Cas9 activity and orthogonality. Mol Cell (2014) 56:333-9. pubmed article See comment in Genetic Engineering & Biotechnology News
  16. Beisel CL, Gomaa AA, Barrangou R. A CRISPR design for next-generation antimicrobials. Genome Biol (2014) 15:516 pubmed article
  17. Gomaa AA, Klumpe HE, Luo ML, Selle K, Barrangou R, Beisel CL. Programmable removal of bacterial strains by use of genome-targeting CRISPR-Cas systems. mBio (2014) 5:e00928-13. pubmed article See comment in Nature, New York Times, Wired Magazine, ScienceNews, Faculty of 1000
  18. Afroz T*, Biliouris K*, Kaznessis Y, Beisel CL. Bacterial sugar utilization gives rise to distinct single-cell behaviours. Mol Microbiol (2014) 93:1093-1103. pubmed article See comment in Mol Microbiol
  19. Beisel CL, Bloom RJ, Smolke CD. Construction of ligand-responsive microRNAs that operate through inhibition of Drosha processing. Methods Mol Biol (2014) 1111:259-67. pubmed article
  20. Afroz T & Beisel CL. Understanding and exploiting feedback in synthetic biology. Chem Eng Sci (2013) 103:79-90. article
  21. Beisel CL, Updegrove TB, Janson BJ, Storz G. Multiple factors dictate target selection by Hfq-binding small RNAs. EMBO J (2012) 31:1961-74 pubmed article See comment in Faculty of 1000
  22. Beisel CL & Storz G. Discriminating tastes: physiological contributions of the Hfq-binding small RNA Spot 42 to catabolite repression. RNA Biol (2011) 8:1-5. pubmed article
  23. Beisel CL & Storz G. The base pairing RNA Spot 42 participates in a multi-output feedforward loop to help enact catabolite repression in Escherichia coli. Mol Cell (2011) 41:286-97. pubmed article See comment in Mol Cell
  24. Beisel CL, Chen, YY, Culler SJ, Hoff KG, Smolke CD. Design of small molecule-responsive microRNAs based on structural requirements for Drosha processing. Nucleic Acids Res (2011) 39(7):2981-94. pubmed article See comment in Faculty of 1000
  25. Beisel CL & Storz G. Base pairing small RNAs and their roles in global regulatory networks. FEMS Microbiol Rev (2010) 34(5):866-82. pubmed article See comment in FEMS Microbiol Rev
  26. Beisel CL & Smolke CD. Design principles for riboswitch function. PLoS Comput Biol (2009) 5(4): e1000363. pubmed article
  27. Bayer TS, Hoff KG, Beisel CL, Lee JJ, Smolke CD. Synthetic control of a fitness trade-off in yeast nitrogen metabolism. J Biol Eng (2009) 3:1. pubmed article
  28. Beisel CL, Bayer TS, Hoff KG, Smolke CD. Model-guided design of ligand-regulated RNAi for programmable control of gene expression. Mol Syst Biol (2008) 4:224. pubmed article See comment in Mol Syst Biol

* multiple first authors, # multiple corresponding authors